The red line is a slightly better indication of your quality of play compared to the green (it should basically run parallel with the green in the long run) - it just takes a quite small chunk of the luck out of the results. You cannot control the red line/green line difference, even if you shove with 23o 1000 times your red line will just get to the long run faster.
For most people it will take about 500 games before you could tell much about your roi, at 500 you can roughly say it +ve or -ve.
The best way to tell if you are making ICM leaks is through using an ICM tool like SnGWizard.
don't worry about EV lines. worry about reviewing your play and being happy with your decisions.
also understand that the results of 50, 100 or even 1,000 games are completely useless. the rule-of-thumb going around a while ago was 5k games was enough of a sample to be relatively confident that you were a winning/losing player but it had a lot of room for error, and 10k games was a pretty good indication of how you were doing in the games - altho even with a 10k sample you're still going to be off from your "true results" a decent amount of the time. There were a few very good winning players who played games they only had 3-4% ROI in and had 10k stretches of breakeven or worse.